c 12281 Search Results


c 12281 u937 cells dsmz acc5 a549 cells dsmz acc107 thp1 cells dsmz acc16 calu 3 cells atcc htb  (DSMZ)
96
DSMZ c 12281 u937 cells dsmz acc5 a549 cells dsmz acc107 thp1 cells dsmz acc16 calu 3 cells atcc htb
C 12281 U937 Cells Dsmz Acc5 A549 Cells Dsmz Acc107 Thp1 Cells Dsmz Acc16 Calu 3 Cells Atcc Htb, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+12281/THP-1/pm40168998-206-197-200
Average 96 stars, based on 1 article reviews
c 12281 u937 cells dsmz acc5 a549 cells dsmz acc107 thp1 cells dsmz acc16 calu 3 cells atcc htb - by Bioz Stars, 2026-09
96/100 stars
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hlmvecs 3000  (ScienCell)
90
ScienCell hlmvecs 3000
High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic in <t>HLMVECs</t> and HDMECs. F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.
Hlmvecs 3000, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+12281/hlmvecs+3000/pmc06080943-227-35-37
Average 90 stars, based on 1 article reviews
hlmvecs 3000 - by Bioz Stars, 2026-09
90/100 stars
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kv4 2 kcnd2 nm 012281 human tagged orf clone  (OriGene)
N/A
KCND2 Myc DDK tagged Human potassium voltage gated channel Shal related subfamily member 2 KCND2
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taf7l hdr plasmid  (Santa Cruz Biotechnology)
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CRISPR/Cas9 KO Plasmids consists of TAF7L-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
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mouse dnah1 activation kit by crispra  (OriGene)
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Dnah1 CRISPRa kit CRISPR gene activation of mouse dynein axonemal heavy chain 1
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plekhm1 nm 001009677 rat tagged orf clone  (OriGene)
N/A
Plekhm1 Myc DDK tagged ORF Rat pleckstrin homology domain containing family M with RUN domain member 1 Plekhm1 10 ug
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kv4 2 kcnd2 nm 012281 human untagged clone  (OriGene)
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KCND2 untagged Human potassium voltage gated channel Shal related subfamily member 2 KCND2
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hey l heyl nm 014571 human untagged clone  (OriGene)
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HEYL untagged Human hairy enhancer of split related with YRPW motif like HEYL
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kif17 nm 001122819 human tagged orf clone  (OriGene)
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Lenti ORF clone of KIF17 Myc DDK tagged Human kinesin family member 17 KIF17 transcript variant 2
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ftsj3 bc012281 mouse tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles Ftsj3 Myc DDK tagged Mouse FtsJ homolog 3 E coli cDNA clone MGC 18657 IMAGE 4012961 200ul 10 7 TU mL
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Image Search Results


High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic in HLMVECs and HDMECs. F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.

Journal: The Journal of Cell Biology

Article Title: Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers

doi: 10.1083/jcb.201706041

Figure Lengend Snippet: High levels of barrier resistance correlate with continuous intercellular junctions and cortical arrangement of the actin cytoskeleton. (A) Confocal images of confluent endothelial monolayers displayed in order of low (HAEC, HBMVEC, and HUVEC) and high levels of barrier resistance (HLMVEC and HDMEC) left to right (bar, 20 µm; representative of n = 3). HLMVEC and HDMEC exhibit a linear and organized junctional staining of VE-cadherin, claudin-5, and ZO-1 compared with an irregular junctional pattern in cell types with lower levels of resistance. Phalloidin staining reveals a strict cortical arrangement of the actin cytoskeleton in HLMVEC and HDMEC, whereas cells with low resistance exhibit more stress fibers. Localization of cortactin is more peripheral and less cytoplasmic in HLMVECs and HDMECs. F-actin fibers aligned along the cell periphery, minimizing radial tension forces at cell junctions, indicated by linear junctional pattern of VE-cadherin/phalloidin overlay images (arrows) and lower presence of pMLC2 in HLMVECs and HDMECs. ( B–F) Graphs presenting mean values of fluorescence intensity across multiple cell–cell junctions per cell type (Fig. S2 B) for each barrier protein shown in A. Junctional components as well as phalloidin and cortactin are concentrated along the cell borders in HLMVECs and HDMECs compared with cell types with low barrier resistance.

Article Snippet: Specifically, we obtained HUVECs (C2519A; Lonza; C-12203; PromoCell), HSaVECs (HSVEC/A; VEC-Technologies; cAP-0019; Angio-Proteomie), HAECs (PCS-100-011; ATCC; 6100; ScienCell), HIAECs (CC-2545; Lonza; cAP-0020; Angio-Proteomie), HBMVECs (ACBRI 376 V; Cell-Systems; cAP-0002; Angio-Proteomie), HUMVECs (C-12295; PromoCell; 7000; ScienCell), HLMVECs (3000; ScienCell; C-12281; PromoCell), HAMVECs (7200; ScienCell), and HDMECs (C-12212; PromoCell; 2000; ScienCell).

Techniques: Staining, Fluorescence

Knockdown of VAV3 reduces barrier strength and alters cytoskeletal arrangement in HDMECs and HLMVECs. (A) siRNA knockdown of three candidate genes with a strong correlation of expression to barrier resistance ( BAIAP2, VAV3, and SORBS2 ) validates the effect of VAV3 as an important regulator. (B) Efficiency of siRNA knockdown for BAIAP2, VAV3, and SORBS2 . (C and E) Effect of VAV3 silencing on barrier resistance compared with combined siRNA knockdown of VAV2 and VAV3 or CLDN5 (positive control) in HDMECs and HLMVECs. (D and F) Efficiency of siRNA knockdown for VAV2 , VAV3 , and CLDN5 (ECIS dataset C and E) in HDMECs and HLMVECs versus control siRNA. (G and H) Bar graphs of barrier resistance levels at 48 h for HDMECs and HLMVECs (ECIS dataset C and E). Error bars show mean ± SEM; *, P < 0.05; n = 3. (I) Table presenting ECIS data modeling values of R b , α , and C m at 48 h for HDMECs and HLMVECs with siRNA knockdown for VAV3 , VAV2/3 , and CLDN5 versus control siRNA, respectively (mean ± SEM; n = 3). (J) Immunofluorescence staining of VE-cadherin, ZO-1, F-actin (phalloidin), and cortactin in HDMEC monolayers subjected to either siRNA knockdown of VAV3 and VAV2/3 or siRNA control (bars, 20 µm). Magnification of phalloidin staining highlights loss of cortical actin and gain of stress fibers upon VAV3 and VAV2/3 knockdown. Translocation of cortactin to the cell periphery (filled arrows) is reduced in monolayers with siRNA knockdown of either VAV3 alone or VAV2/3 (open arrows). (K) Fluorescence intensity across cell–cell junctions (mean of n = 6) in cells exposed to siRNA control, siRNA VAV3 , and siRNA VAV2/3 (for VE-cadherin, ZO-1, phalloidin, and cortactin; as shown in J).

Journal: The Journal of Cell Biology

Article Title: Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers

doi: 10.1083/jcb.201706041

Figure Lengend Snippet: Knockdown of VAV3 reduces barrier strength and alters cytoskeletal arrangement in HDMECs and HLMVECs. (A) siRNA knockdown of three candidate genes with a strong correlation of expression to barrier resistance ( BAIAP2, VAV3, and SORBS2 ) validates the effect of VAV3 as an important regulator. (B) Efficiency of siRNA knockdown for BAIAP2, VAV3, and SORBS2 . (C and E) Effect of VAV3 silencing on barrier resistance compared with combined siRNA knockdown of VAV2 and VAV3 or CLDN5 (positive control) in HDMECs and HLMVECs. (D and F) Efficiency of siRNA knockdown for VAV2 , VAV3 , and CLDN5 (ECIS dataset C and E) in HDMECs and HLMVECs versus control siRNA. (G and H) Bar graphs of barrier resistance levels at 48 h for HDMECs and HLMVECs (ECIS dataset C and E). Error bars show mean ± SEM; *, P < 0.05; n = 3. (I) Table presenting ECIS data modeling values of R b , α , and C m at 48 h for HDMECs and HLMVECs with siRNA knockdown for VAV3 , VAV2/3 , and CLDN5 versus control siRNA, respectively (mean ± SEM; n = 3). (J) Immunofluorescence staining of VE-cadherin, ZO-1, F-actin (phalloidin), and cortactin in HDMEC monolayers subjected to either siRNA knockdown of VAV3 and VAV2/3 or siRNA control (bars, 20 µm). Magnification of phalloidin staining highlights loss of cortical actin and gain of stress fibers upon VAV3 and VAV2/3 knockdown. Translocation of cortactin to the cell periphery (filled arrows) is reduced in monolayers with siRNA knockdown of either VAV3 alone or VAV2/3 (open arrows). (K) Fluorescence intensity across cell–cell junctions (mean of n = 6) in cells exposed to siRNA control, siRNA VAV3 , and siRNA VAV2/3 (for VE-cadherin, ZO-1, phalloidin, and cortactin; as shown in J).

Article Snippet: Specifically, we obtained HUVECs (C2519A; Lonza; C-12203; PromoCell), HSaVECs (HSVEC/A; VEC-Technologies; cAP-0019; Angio-Proteomie), HAECs (PCS-100-011; ATCC; 6100; ScienCell), HIAECs (CC-2545; Lonza; cAP-0020; Angio-Proteomie), HBMVECs (ACBRI 376 V; Cell-Systems; cAP-0002; Angio-Proteomie), HUMVECs (C-12295; PromoCell; 7000; ScienCell), HLMVECs (3000; ScienCell; C-12281; PromoCell), HAMVECs (7200; ScienCell), and HDMECs (C-12212; PromoCell; 2000; ScienCell).

Techniques: Knockdown, Expressing, Positive Control, Control, Immunofluorescence, Staining, Translocation Assay, Fluorescence